You will learn how proteins are the cellular manifestation of genetic information. They are assembled into a polymeric structure from monomers derived in part from components in our diet. Proteins catalyze metabolic reactions, replicate DNA, respond to stimuli, provide movement, and much more. Using video lectures, articles, case studies, and molecular models, we will explore how proteins are constructed, how they fold into 3-dimensional shapes, the kinds of bonds that hold these folded structures together, and the immense range of roles that proteins assume ‑ from structural proteins found in muscle to catalysts for cellular chemical reactions.

Purification and characterization are essential to understand protein structure and function, and we will identify a variety of methods to uncover how these tiny machines drive almost all living processes.

How proteins are structured and fold into functional forms
The variety of functions that proteins perform, including enzyme catalysis
How to purify, analyze, and characterize protein structure and function

Syllabus

Lecture 1: Protein Structure

Introduction tocentral concepts important for understanding biological molecules, components of proteins, and discovering how these components determine a protein's active, dynamic form.

Lecture 2: Protein Function

Examination of structure and function of the various classes of proteins, how shape determines function, how binding small molecules is important for function, and the nature of transport proteins that move materials

Lecture 3: Protein Function and Purification

Exploration of the dynamic nature and exquisite specificity of enzymes and the methods that can be used to purify a specific protein.

Lecture 4: Protein Characterization

Understanding methods that determine protein concentration, assess molecular weight, determine structure, and identify contribution to cell function.